Design of Superparamagnetic Iron Oxide Nanoparticle for Purification of Histidine- Tagged Recombinant Proteins
نویسندگان
چکیده
We report a novel purification system for 6 His-tagged proteins by magnetic affinity separation. We have developed superparamagnetic Fe3O4@SiO core-shell particles with immobilized surface iminodiacetate groups to chelate with Ni. This Ni magnetic silica nanoparticle has been shown as an efficient carrier, binder and anchor to obtain his-tagged protein directly from total cell lysate. The structural characteristic of the powders, the size of the particle and aggregates in the colloid and magnetic property have been studied by XRD, HRTEM, FTIR, XPS and VSM studies. Due to high efficiency, costeffectiveness, bio-compatibility and versatility, these magnetic-silica nanoparticles with specific affinity offered by metal chelation may provide new possibilities in biotechnological applications.
منابع مشابه
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